Penstemon caryi (Scrophulariaceae) is an endemic of the Big Horn Mountains of Wyoming and the Pryor Mountains of Montana. The species is endemic to exposed limestone outcrops that occur infrequently throughout the region. Nine populations were collected from The Nature Conservancy's Tensleep Preserve in Wyoming. Five inter-simple sequence repeat (ISSR) markers were used to evaluate genetic diversity found within and among populations of P. caryi. Results from this study, in conjunction with results from a reproductive ecology study, will be used to determine conservation strategies for the species on Nature Conservancy lands.

Key words: Conservation Genetics, Genetic Diversity, ISSR, Penstemon caryi, Population Genetics