MüLLER1, KAI*, THOMAS BORSCH1, LAURENT LEGENDRE2, STEFAN POREMBSKI3, AND WILHELM BARTHLOTT1. 1Botanisches Institut und Botanischer Garten, Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 170, 53115 Bonn, Germany; 2Laboratoire de Biologie et Physiologie Vegetales, Universite de Reims Champagne-Ardenne, Moulin de la Housse - B.P. 1039, 51687 Reims Cedex 2, France; 3Institut für Biodiversitätsforschung, Allgemeine und Spezielle Botanik, Universität Rostock, Wismarsche Straße 8, 18051 Rostock, Germany. - A phylogeny of Lentibulariaceae based on sequences of matK and adjacent noncoding regions.
The carnivorous angiosperm family Lentibulariaceae comprises about 280
species in 3 genera (Utricularia, Pinguicula, Genlisea) and is
of nearly worldwide distribution. It belongs to the order Lamiales
within euasterids. From representatives of Lentibulariaceae and
different outgroup taxa of the Lamiales the whole trnK intron
including the matK gene was sequenced. The size of the
matK coding region in Lentibulariaceae ranges between 1521 and
1581 bp. Size differences are caused by indels from 3 - 36 bp, which
accumulate in the first half of the gene. The length of the noncoding
region adjacent to the 5´ end of matK is 729 - 780 bp, and of
the region adjacent to the 3´ end 305 - 330 bp. Sequence variability
within Lentibulariaceae is 23% for the coding region as compared to
27% for the noncoding region. For the large 5´ noncoding region a
robust alignment was obtained, whereas the short 3´ region could not
be aligned all over due to high numbers of indels. Parsimony analyses
based on substitutions and carried out independently for the
matK gene and the long 5´noncoding region yielded identical,
well resolved phylogenies with high bootstrap and decay support. The
matK indels appear to be highly informative phylogenetically
and seem to be much less homoplastic than substitutions.
Lentibulariaceae appear monophyletic and Byblis is resolved as
closest relative. Within Lentibulariaceae, Pinguicula diverges
first and its species appear as a terminal clade on a long branch.
Genlisea is sister to Utricularia with quite similar
branch-lengths within the topology of the
Genlisea-Utricularia-clade.
Key words: carnivorous plants, Lentibulariaceae, matK, molecular systematics, trnK intron