WOLFE, ANDREA D. Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, Columbus, OH 43210. - Using ISSR markers in studies of natural populations.
Inter-simple sequence repeat markers are generated from single-primer
PCR reactions where the primer is designed from di- or trinucleotide
repeat motifs with a 5'- or 3'-anchoring sequence of one to three
nucleotides. The amplified regions represent the nucleotide sequence
between two SSR priming sites oriented on opposite DNA strands. The
premise is that SSR regions are scattered evenly throughout the genome
and the chances of amplifying between two adjacent regions within the
limits of Taq Polymerase processivity is high enough that a large
number of polymorphic bands should be generated. ISSR markers are
inherited in a dominant or codominant Mendelian fashion. They are
interpreted as dominant markers similar to RAPD data. The absence of
a band is interpreted as primer divergence or loss of a locus through
the deletion of the SSR site or chromosomal rearrangement. The
utility of ISSR markers has recently been demonstrated for use in
studies of natural populations for a range of applications from
population-level to interspecific studies. The objective of this
workshop is to introduce researchers into the use of ISSR markers for
studies of natural populations. An overview of the method will be
given with an emphasis on data interpretation and analysis.
Key words: DNA techniques, ISSR markers, molecular biology, workshop